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Mediomics品牌——用于檢測大分子物質的技術

更新時間：2022-12-26 點擊次數：959

Mediomics公司于2001年創立于美國密蘇里州，Mediomics已通過ISO 9001: 2015認證并致力于為科研機構、即時檢測、醫藥企業以及醫療和環境研究市場提供創新的檢測試劑盒和生物傳感器等產品，其檢測試劑盒可以簡單快速的定量檢測生物學和臨床相關的大分子物質，包括DNA、生物標志物、激素、抗體、細胞內信號分子、蛋白質復合物、細菌、真菌和病毒等。

Mediomics代理——上海起發

Mediomics品牌——用于檢測大分子物質的技術

Mediomics目前擁有四個核心專有技術：

1. Bridge-It：一種快速靈敏的DNA-蛋白質結合物檢測平臺。

Bridge-It系列試劑盒可用來檢測DNA結合蛋白及其配體，和共調節因子。該系列試劑盒基于一個簡單的實驗原理：一段針對已知蛋白的具有序列特異DNA結合位點的DNA雙鏈被分為兩段，每段含有一個短的單鏈突出端。這些突出端很短，因此沒有靶蛋白時他們不會自發的重新結合。當靶蛋白出現時，靶蛋白與完整的DNA序列結合位點的高親和力使得兩段DNA雙鏈重新結合。通過在每段位點加入適當的熒光探針就可以檢測到該結合。然后用熒光共振能量轉移技術（fluorescence resonance energy transfer，FRET）檢測熒光信號的變化來判斷DNA結合蛋白或其配體。

該系列試劑盒具有以下特色：
1)簡單的混合和測試方法：只需要混合樣本和反應試劑，不需要其他操作技術。直接檢測樣本無需稀釋步驟，只需混合樣本/標準品和反應試劑。2)快速：孵育30min后即可檢測熒光信號。3)定量檢測。4)可重復性好：實驗變異性小，避免了抗體或放射性探針帶來的批次間的差異。5)可實現高通量篩選。

2. PINCER：一種快速的生物傳感器、生物測定和生物芯片平臺。

PINCER Assays是一種用于定量檢測生物樣本中的生物標志物、細胞和病原體濃度的技術。該系列產品分為FRET-PINCER Assay和TRF-PINCER Assay兩種，分別使用熒光共振能量轉移技術（fluorescence resonance energy transfer，FRET）和時間分辨熒光（Time-Resolved Fluorometry，TRF）方法檢測。其檢測原理是：帶有熒光探針的抗體由一個靈活的連接物通過互補的單鏈DNA突出端與DNA雙鏈化學結合，這些突出端使得兩個DN**段有結合的可能性，然而在沒有靶蛋白的情況下，兩個DNA片段結合的可能性極小。當存在靶蛋白時，與PINCER試劑結合的靶蛋白將促使互補DNA單鏈突出端的結合而形成雙鏈DNA。這種緊密穩定的結合使熒光團聚合起來，發生熒光共振能量轉移。因此，檢測樣本發出的熒光能量水平或亮度的變化即可定量靶蛋白的濃度。

PINCER Assay系列試劑盒的特色：

1)簡便：混合樣本/標準品與分析試劑在室溫孵育。

2)快速：孵育30min后檢測熒光信號。

3)檢測范圍廣：直接檢測樣本，無需稀釋步驟。

4)兼容性：與大多數熒光讀數儀兼容。

5)可實現高通量篩選。

3、使用創新技術篩選和研發可用于科研、臨床診斷和治療的新型高親和力捕獲試劑。

4、抗血管生成相關療法。

這些技術已被證實適于檢測一系列關鍵大分子物質，包括cAMP、色氨酸、S-腺苷蛋氨酸、磷酸二酯酶、生物素、胰島素、C肽、白蛋白、C反應蛋白、IgG、IgM和大腸桿菌等等。

Mediomics相關產品

Product	Catalog Number	Kit components
Bridge-It Assay Kits
Bridge-It cAMP 384	122938	10uM cAMP stock standard in H2O, 2X Assay Solution A, 10X Lysis Buffer, Buffer B
Bridge-It cAMP 100	122939	10uM cAMP stock standard in H2O, 2X Assay Solution A, 10X Lysis Buffer, Buffer B
Bridge-It cAMP 96	122935	10uM cAMP stock standard in H2O, Assay Solution A, 10X Lysis Buffer, Buffer B, 10X KRB-IBMX Buffer
Bridge-It cAMP 50	122934	10uM cAMP stock standard in H2O, Assay Solution A, 10X Lysis Buffer, Buffer B, 10X KRB-IBMX Buffer
Bridge-It cAMP-PDE 384	PD1007-384	10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B
Bridge-It cAMP-PDE 100	PD1007-100	10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B
Bridge-It cAMP-PDE 96	PD1016-96	10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B
Bridge-It cAMP-PDE 60	PD1016-60	10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B
Bridge-It SAM 384	1-1-1004B	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
Bridge-It SAM 100	1-1-1004A	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
Bridge-It SAM 96	1-1-1003B	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
Bridge-It SAM 50	1-1-1003A	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
Bridge-It L-MET 384	1-1-1006B	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
Bridge-It L-MET 100	1-1-1006A	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
Bridge-It L-MET 96	1-1-1005B	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
Bridge-It L-MET 50	1-1-1005A	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
Bridge-It L-Tryptophan 384	1-1-1002B	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
Bridge-It L-Tryptophan 100	1-1-1002A	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
Bridge-It L-Tryptophan 96	1-1-1001B	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
Bridge-It L-Tryptophan 50	1-1-1001A	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
TR-FRET Bridge-It Assay Kits
TR-FRET Bridge-It® L-Tryptophan 100	TRF-1-1-1002A	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
TR-FRET Bridge-It® L-Tryptophan 384	TRF-1-1-1002B	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
TR-FRET Bridge-It® L-Tryptophan 50	TRF-1-1-1001A	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
TR-FRET Bridge-It® L-Tryptophan 96	TRF-1-1-1001B	1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C
TR-FRET Bridge-It® SAM 100	TRF-1-1-1004A	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
TR-FRET Bridge-It® SAM 384	TRF-1-1-1004B	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
TR-FRET Bridge-It® SAM 50	TRF-1-1-1003A	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
TR-FRET Bridge-It® SAM 96	TRF-1-1-1003B	10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM
TR-FRET Bridge-It® L-MET 50	TRF-1-1-1005A	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
TR-FRET Bridge-It® L-MET 96	TRF-1-1-1005B	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
TR-FRET Bridge-It® L-MET 100	TRF-1-1-1006A	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
TR-FRET Bridge-It® L-MET 384	TRF-1-1-1006B	L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution
FRET-PINCER Assay Kits
hAlbumin FRET 384	AH1005-384	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin FRET 100	AH1005-100	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin FRET 50	AH1005-50	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin FRET 96	AH1005-96	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP FRET 384	CH1008-384	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP FRET 100	CH1008-100	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP FRET 96	CH1021-96	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP FRET 50	CH1021-50	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin FRET 384	IH1010-384	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin FRET 100	IH1010-100	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin FRET 50	IH1017-50	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin FRET 96	IH1017-96	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
Mouse IgM FRET 384	IM1009-384	Mouse IgM standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
Mouse IgM FRET 100	IM1009-100	Mouse IgM standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
Mouse IgG FRET 384	MIG1012-384	Mouse IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
Mouse IgG FRET 100	MIG1012-100	Mouse IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
rIgG FRET 96	RI1024-96	Rabbit IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
rIgG FRET 50	RI1024-50	Rabbit IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
rIgG FRET 384	RI1015-384	Rabbit IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
rIgG FRET 100	RI1015-100	Rabbit IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
TRF-PINCER Assay Kits
Biotin TRF 384	BTTR-1006-384	Biotin standard (2 uM), PINCER A, PINCER B, Assay Solution C, Buffer M
Biotin TRF 100	BTTR-1006-100	Biotin standard (2 uM), PINCER A, PINCER B, Assay Solution C, Buffer M
hAlbumin TRF 384	AHTR1005-384	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin TRF 100	AHTR1005-100	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin TRF 50	AHTR1005-50	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hAlbumin TRF 96	AHTR1005-96	Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP TRF 50	CHTR1021-50	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP TRF 96	CHTR1021-96	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP TRF 100	CHTR1008-100	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hCRP TRF 384	CHTR1008-384	hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hIgG TRF 384	HITR1011-384	hIgG standard (40 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hIgG TRF 100	HITR1011-100	hIgG standard (40 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin TRF 384	IHTR1010-384	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin TRF 100	IHTR1010-100	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin TRF 50	IHTR1017-50	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
hInsulin TRF 96	IHTR1017-96	Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgG TRF 384	MIGTR1012-384	Mouse IgG standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgG TRF 200	MIGTR1012-200	Mouse IgG standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgG TRF 96	MIGTR1020-96	Mouse IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgG TRF 50	MIGTR1020-50	Mouse IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgM TRF 384	IMTR1009-384	Mouse IgM standard (80 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
mIgM TRF 200	IMTR1009-200	Mouse IgM standard (80 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M
Other Products
Recombinant human methionine aminopeptidase-2	PM-803262-100 µg	Each vial contains 200 µl (~100 µg) of purified human methionine aminopeptidase-2 in 20 mM Hepes, pH 7.4, 0.15 M NaCl, 0.02% NaN3, 10% glycerol and 0.1 mM Co(II)
Anti-recombinant human MetAP-2 (full-length) Polyclonal Antibody	PAB-503208	Each vial contains 120 µl of anti-rhMetAP2 IgG (0.85 mg/ml) in TBS with 0.02% NaN3
Anti-hMetAP-2 (C-terminal) Polyclonal Antibody	PAB-503209	Each vial contains 100 µl of anti-hMetAP2 IgG in PBS
10X KRB-IBMX Buffer	122937	10X KRB-IBMX Buffer solution is a mixture of a 10X concentrate of Krebs-Ringer Bicarbonate Buffer (KRB) supplemented with a 10X concentration (i.e., 7.5 mM) of 3-Isobutyl-1-Methylxanthine (IBMX) and containing 10% ethanol (for use in cAMP Assays, order with cAMP assay kits)

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